Composite

Part:BBa_K322128

Designed by: Chris French and Richard Partridge Hicks   Group: iGEM10_Edinburgh   (2010-10-08)


Phycocyanobilin synthesis genes + Red light detection system (EYFP as reporter)

There are some problems with the original coliroid parts that are in the 2010 distribution. We resubmitted this in pSB1C3, with the reporter system attached. This part contains the phycocyanobilin synthesis genes. PCB binds to cph8 and is required for light detection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None